Attended Dr. Li-Shu Wang's team to investigate the prevention of black raspberries on pancreatic cancer.
1. Breeding mice to create new models:
(1) To generate double and triple transgenic mice: Gene mutations of K-ras and p53 in pancreatic model.
(2) Bioluminescent Orthotopic Model of Pancreatic Cancer: Luciferase-tagged pancreatic cancer cells are resuspended in Matrigel and delivered into the pancreatic tail during laparotomy.
2. Bioluminescent in vivo imaging system (IVIS): To detect Panc-1-Luc+ cells migration.
3. Flow cytometer to measure the population/function of immune cells.
4. Immunohistochemistry of Pancreatic Neoplasia.
5. Attended American Association for Cancer Research (AACR) in 2014.
6. Journal presentation and progress report.
7. Involved in several journals and manuscript publication.
(1) Black raspberries suppress colonic adenoma development in ApcMin/+ mice: relation to metabolite profiles. Carcinogenesis 2015 Oct;36(10):1245-53.
(2) Systemic Metabolite Changes in Wild-type C57BL/6 Mice Fed Black Raspberries. Nutr Cancer 2017 Feb-Mar;69 (2):299-306.
(3) Can dietary interventions enhance cancer immunotherapy through manipulating gut microbiota in humans?
eFood-2020-0025.
Cell Therapy included engineered patient's immune stem cells to recognize and attack their target cancer cells. 1. Chimeric Antigen Receptor (CAR) T cells
(1) Before the initiation of the CAR-T therapy, using IHC staining to find the specific target antigen.
(2) Optimization of manufacturing conditions for CAR-T cells for autologous adoptive immunotherapy.
(3) I have been used simple approach to transduce primary human T cells with CAR.
(4) In vitro co-culture method to evaluate cytotoxicity CAR-T with cancer cells.
2. Adipose-derived Stem Cells
1. I have used transgenic-modified primary adipose-derived stem cells models to study the mechanism of the cytotoxic of the cancers and cirrhosis.
2. Flow cytometry, LDH-Glo or Delphia to investigate the potential killing effects of the transgene on the of liver cirrhosis and cancers.
3. Setup SOP
4. Apply ADSC proposal to Taiwan Food and Drug Administration (TFDA).
Involved in Dr. Shu-Hong Wang's lab for Spinal Muscular Atrophy (SMA) study in 2008.
1. I built up my strong experimental skills in SHW's lab by training from 8:00-0:00.
2. Obtained the scholarship in the university
3. I accomplished simple thesis to include my three years studies.
4. I went through the Recommendation-Selection Admission Program to National Cheng Kung University as a graduate student.
Involved in Dr. Mei-Ling Tsai's lab for Cardiovascular study in 2011. Dissertation: Activated ERbeta increases the expression of L-type Ca2+ channel and enhances the hypertrophic phenotype of neonatal rat hearts
1. Had been trained to use English to present and write our proposal and thesis.
2. Trained medical students in Physiology experiments.
3. 2012 Presented my studies in the 27th Joint Annual Conference of Biomedical Science.
4. 2012 Went to Hong Kong for academic exchange with the students in the Chinese University of Hong Kong (CUHK).
5. 2013 Won Academic Excellence Award for my dissertation.
1. Transgenic mice mating
2. Mating: mice/ rats
3. Ear punch
4. IV injection
5. Subcutaneous injection
6. Intraperitoneal
7. IVIS system: luciferase assay
8. Take care of myocardial infarction rat model
9. Laparotomy
10. Anesthesia: Isoflurane
1. HE staining_rat heart model
2. IHC staining: Cancer patient's tumors
3. Toluidine blue O: ADSC differentiate into chondrocyte