Tsunghan Hsieh

Motivated, results-oriented Data Scientist specializing in biomedicine with strong data science skills.

• 6+ years of experience in conducting transcriptomes and TF-binding sequences in high-performance computing (HPC) environment using shell-scripting.
• Adept in analyzing and visualizing high-dimensional RNA-seq data using various computer softwares (e.g., Python, R etc.) •Adept in analyzing T cells with flow cytometry.
• Good project management skills and documenting reports/papers for research projects.
• Great communication skills and actively attending conferences for presenting, discussing and learning novel climate science research and technology.
• Team player working on interdisciplinary projects with solid problem solving skills and a passion for applying data science to solve challenging problems in business.

Okinawa, Japan

Relevant working experience

PhD candidate • Okinawa Institute of Science and Technology Graduate University

# Project: Decipher the role of AP-1 transcription factor JunB in CD4+ T cells
- Analyzed data of RNA-seq and ChIP-seq to predict JunB target genes.
- Built a data analysis workflow for identifying genes bound and regulated by a given transcription factor in HPC environment with Shell scripts and R.
- Performed isolation and culturing of primary cells, functional cellular assays, immune phenotyping by FACS, qPCR, ELISA.
- Performed in vivo studies in infection, and autoimmune model.
- https://github.com/oistishikawa/

2015 - present

Product manager • Personal Genomics Inc.

- Developed a IC-based, next generation DNA sequencer (NGS).
- Designed and tested a new method for single-molecule DNA library preparation.
- Collaborated with faculty from National Taiwan University to deliver single-molecule DNA into our platform.

2013 - 2015

Sales • Taiwan ELGA

- Successfully sold 6 instruments within one year under a fierce competition from Merck Millipore.
- Successfully sold products to clients 3 months after entering the company.
- Conducted cold call and joint call to develop clients.

2012 - 2013

qPCR R&D assay • GeneReach Biotechnology corporation

- Developed qPCR based IVD products for tuberculosis (TB) detection.

2008 - 2009

Instrument and software

FlowJo. GraphPad Prism. Fortessa. qPCR. ELISA. R. NGS data analysis. FACS. Flow cytometry analysis. Illumina. 10X Genomics.

Education

2015-2021, Okinawa Institute of Science and Technology Graduate University, PhD Immunology
2006-2008, National Yang Ming University, MSc Immunology
2000-2004, National Yang Ming University, Bachelor, Life Science

Publish:
1. Hsieh, TH., Sasaki, D., Taira, N., Sarkar, S. et al. JunB regulates the common and lineage-specific transcriptional program of distinct CD4+ T cells. In progress (2021).
2. Taira, N., Sarkar, S., Koizumi, Si., Hsieh, TH. et al. JunB is indispensable for the sustenance of antigen specific CD8+ T cells. In progress (2021).

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3. Koizumi, Si., Sasaki, D., Hsieh, TH. et al. JunB regulates homeostasis and suppressive functions of effector regulatory T cells. Nat Commun 9, 5344 (2018).

Reference: (Dr. Ishikawa, PI) [email protected]; (Dr. Lee, CEO of Personal Genomics) [email protected]