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2. Ruff Ryders Anthem ft. Eminem, Obie Trice, Kokane, Dru Down, & Pusha T 3:35 3. Suicidal Thoughts 5:24 4. For My Downfalls 5:33 5. So Far So Good 6:18 6. Can I Play With Your Pussy? ft. Big T 6:50 7. I Can Play With Your Pussy Too ft. Big T 6:18 8. Forever 4:03 9. Can't Go Wrong 5:02 10. Hot Patootie ft. Big T, Camron, Kenna, & Obie Trice 4:55 11. Hot & Sexy (Interlude) 3:41 12. It's So Nice 4:04 13. Kill Bill 2:38 14. Bonafide 4:01 15. It's So Nice (Interlude) 3:51 16. Sex Is Back In 4:36 17. Suicidal Thoughts (Interlude) 2:55 18. Ruff Ryders Anthem (Interlude) 1:28 19. For My Downfalls (Interlude) 3:13 20. Suicidal Thoughts (Interlude) 1:40 21. Da Introduction (Interlude) 0:51 22. Sex Is Back In (Interlude) 3:25 23. I'll Still Love You (Interlude) 3:25.Detection of Escherichia coli O157:H7, Salmonella typhimurium, Listeria monocytogenes, and Clostridium perfringens by epifluorescence microscopy. Detection of foodborne pathogenic bacteria by epifluorescence microscopy has been developed. Escherichia coli O157:H7, Salmonella typhimurium, Listeria monocytogenes, and Clostridium perfringens cells were stained with a commercial antiserum and heat-killed cells were used as a negative control. E. coli O157:H7, S. typhimurium, and L. monocytogenes cells stained with the antiserum could be detected by epifluorescence microscopy using filters that provide excitation and emission wavelengths specific for fluorescein. However, in contrast to previous studies, the antiserum reacted with heat-killed L. monocytogenes cells and produced a diffuse fluorescent background. This background was removed by using filters that excite and emit fluorescein. The antiserum

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