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.buku_fisih_muamalah.pdf File buku_fisih_muamalah bagep.buku_fisih_muamalah.pdf PDF arahan kini.pdf.adhan_kini.pdf Download PDF Print or Save ) P = + Δ The time interval for each of the PR phases is as follows: t ( N / * where, *P* is the period of the exponential growth phase, *N* is the number of cells in the colony, and *t* is the time interval between each measurement. The time required to reach a certain time interval is determined using [equation (4)](#FD4){ref-type="disp-formula"}: . Equation (5) can be rewritten as follows: The PR time interval is defined as the interval required to reach 99.9% of the final number of cells. 2.5. Cell growth analysis ------------------------- The *S. cerevisiae* strains with *E. coli* genes were cultured in the MB liquid medium. The OD of each *S. cerevisiae* strain was measured at an optical density of 600 nm using a UV-visible spectrophotometer (Lambda 20, PerkinElmer, Waltham, MA, USA) at 30 °C with shaking (150 rpm) for 24 h. 2.6. Determination of the metabolic rates of *S. cerevisiae* strains -------------------------------------------------------------------- The initial oxygen concentration was determined by a Winkler's method and determined by an oxygen sensor. The oxygen concentration was measured for 5 s at 30 s intervals for the initial 5 min using an Eppendorf Biospectrometer 522. For each *S. cerevisiae* strain, one milliliter of the MB liquid medium was placed in a 15 mL Falcon tube with a

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