生物科技研發人員、醫藥研發人員、生物學專業與研究人員、助理研究人員、研究助理
Ming Ren Yang Associate Scientist Experience Associate Scientist• AIBIOS K.K. JunJunHelp to set up a new share lab in National Biotechnology Research Park, Nangang. Assisting in the development of new drugs and experimental ideation, immune cell culture, and the establishment of NK cell activity functional testing. Research Associate • ARCE NovMarPlasmid construction Research Associate • Institute of Biological Chemistry, Academic Sinica AugJunAssisting in advancing the progress of laboratory experiments, basic biochemical/molecular biology experimental operations, including qPCR, Western blotting, plasmid construction, antibody purification, lentivirus production, CRISPR gene knockout, cell culture, etc., and participating in the execution of projects under the National Health Institute. EducationNational Taiwan University Biochemical Sciences MasterNational Yang Ming Chiao Tung University Department of Life Science Biology major and research In addition to basic molecular biology and cell biology experiments (such as Western blotting, qPCR, cell culture, etc.), I have also participated in animal experiments involving antibody preparation and the study of molecular mechanisms. I also have experience in culturing and differentiating placental stem cells and published a related research paper in the Journal of Biological Chemistry inApart from designing experimental plans, I have also been involved in writing research papers and project applications (National Health Institute projects). After joining AIBIOS K.K. , I have not only expanded my expertise in immune cell culture and flow cytometry operation but have also contributed to the establishment of drug functional testing methods. SKills 1. Protein analysis: protein pull-down, Western blotting, BCA assay, ELISA, etc. 2. Virus packaging and collection: Preparation of lentivirus, retrovirus, and Zika virus in small quantities. 3. Plasmid construction 4. RNA-related analysis: RNA purification, qPCR, preparation of RNA sequencing samples. 5. Cell culture: Placental cells, placental stem cells, and immune cell culture. 6. Gene editing: CRISPR/Cas9 gene knockout. 7. Polyclonal antibody preparation and purification: Preparation of antigen peptides, experimental animal antigen injection, experimental animal blood collection, and serum separation.I wrote the thesis for this degree. The results for these years also published in Journal of Biological Chemistry inLink: https://pubmed.ncbi.nlm.nih.gov//). 8. Flow ...