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Ying Tung Lee(Tess)


Taoyuan, TW
Email: [email protected]

Phone: 0970-718-200

SKILLS & RESEARCH EXPERIENCE


Laboratory skills

Cell culturing and sterile technique │ Migration & Invasion│ MTT│ PCR │RT-qPCR│ Ultracentrifugation │ Molecular cloning│PBMC isolation │DNA, RNA isolation & purification │ IHC │ Immunoprecipitation│ Western Blotting │ Lentivirus packaging│ Gel electrophoresis│SDS-PAGE │ Colony PCR │ Plasmid isolation │ Transformation │ Gram-staining


Data handling & IT

GraphPad Prism 7 │ BLAST search│ MS Word│ Excel│ Powerpoint


Research Assistant│Chang Gung University, Taiwan

Aug 2020 ~ Present

• Project 1: Studied a long non-coding RNA (lncRNA) X  in Taiwanese patients with colorectal cancer. 

• Project 2: Studied EMT by treating exosome in established cell lines. 

• Successfully generated stable cell line by lentivirus transduction.

• Performed cell-based assays and tests to functionally characterize CRC-specific regulation of the lncRNA X in CRC carcinogenesis.

• Mechanistic dissection of lncRNA-mediated gene and signaling pathways in CRC tumorigenesis.

• Executing accurate and timely record keeping and data management for each test undertaken.

• Training and supervising students for basic experiments.

• Assisted in subcutaneous injection of cancer cells in mice.

• Assisted in administration of drug Intraperitoneally in mice.

Clinical Research Assistant│Linkou Chang Gung Memorial Hospital, Taiwan

Dec 2019 ~ Aug 2020

• Processed human blood samples of STEMI patients, including DNA, RNA and PBMC isolation.

• Recrutied study participants

• Provided clinicians with instructions on how to conduct the trials.

• Reviewed literatures to analyse flow cytometry data.

• Ensured studies are conducted in accordance with ICH GCP and the guidelines of the Institutional Review Board (IRB).

• Coordinated operations with different departments, including communication with site supervisors.

• Managed information database of more than 500 patients.

Final Year Project│The Christie NHS Foundation Trust, UK                            Feb ~ Apr 2019

Project title: "An investigation on the expression of caspase-X in DCIS and IDC in well-characterised patient cohorts".

Key outcomes: 

• Identified of breast cancer biomarkers in 261 biopsy samples.

• Development of an IHC protocol using caspase-X on the BenchMark ULTRA IHC/ ISH system.

• Organised of patient records and inputting the details onto databases.
• Analysed experimental data.

• Practise of strict health and safety procedures in the laboratory, using the correct equipment appropriately and according to the task.
Supervisor: Prof Nigel Bundred MD, Fellow of the Royal Colleges of Surgeons (FRCS).

Summer Research Intern│Academia Sinica, Taiwan                                           Jul  ~ Aug 2018

• Worked in a research environment studying ADAR genes in Amyotrophic lateral sclerosis (ALS) patients.
• Successfully used molecular cloning to generate recombinant DNA for later study.
• Carried out batch culture of bacteria, DNA isolation and purification.
• Performed routine lab protocols such as PCR, gel electrophoresis, preparing culture media and Western blotting. 

• Obtained and organized information from scientific papers. 
Supervisor: Dr Yi-Ying Wu.

EDUCATION

BSc Hons in Biomedical Sciences│The University of Manchester, UK               2016 ~ 2019

Class: 2:1

Relevant modules and grades:

• 1st class marks in laboratory-based units: Genetics Research Skill Module (79%) and Human Sciences Experimental Design Module (78%).

• 1st class mark for Dissertation: "An investigation of stem cell therapy in treating Diabetes Mellitus and Parkinson's disease" (75%).

• 1st class marks for final year Literature Review: "Biomarkers and the therapeutic uses in treating breast cancer stem cells" (85%) and Laboratory Research Project - "An investigation on the expression of caspase 14 in breast ductal carcinoma in situ and ductal invasive carcinoma in well characteristed patient cohorts" (74%).

Strong lecture unit marks, including: Toxin, Toxicants and Toxicity (72%), 'Omic Technologies and Resources (68%), Computational Approaches to Biology (64%) and Disease in Nature (63%).

GCE A LevelsBungay High Sixth Form, UK                                                              2014 ~ 2016

Mathematics (A), Biology (B), Chemistry (B)

ADDITIONAL SKILLS & WORK EXPERIENCE


Languages: English (Proficient - language of study)│ Cantonese (Native) │ Mandarin (Native) 

Written communication skills developed through completing a dissertation, literature review and and writing up numerous laboratory projects. 

Completed GCP and IRB courses provided by Center for Taiwan Academic Research Ethics Education.

Full clean driving licence. 

Marketing Officer│ UoM Macau Society, UK                                              Sep 2017 ~ Jun 2018

• Promoted events and gatherings through social media.
• Assisted in coordinating events and recruiting new members.

• Enhanced spoken communication skills by interacting with colleagues and members. 

• Acquired multi-tasking and problem-solving skills.

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