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Avatar of Tai Yuan Hong.
Offline
Avatar of Tai Yuan Hong.
Offline
Research Assistant @National Yang Ming Chiao Tung University
2021 ~ 2022
Researcher
Within one month
CTLA4Ig as a safe and effective treatment for collagen-induced arthritis Jiang FY*, Zhang YZ * , Tai YH*, Chou CY, Hsieh YC, Chang YC, Huang HC, Li ZQ, Hsieh YC, Chen IJ, Huang BC, Su YC, Lin WW, Lin HC, Chao JI, Yuan SF, Wang YM, Cheng TL, Tzou SC. (*Fu-Yao Jiang, Yan-Zhu Zhang, and Yuan-Hong Tai contributed equally to this study) Inflammation and Regeneration 2023 Feb 16;43(1):13. doi:/sSkills Recombinant protein engineering Animal experiment skills Microsoft Office and Photoshop Graphpad Prism software Language English - Fairly Good Chinese - Fluent Malay - Fairly Good
Research Skills
Data Analysis
Molecular Cloning
Employed
Open to opportunities
Full-time / Interested in working remotely
4-6 years
國立陽明交通大學(National Yang Ming Chiao Tung University)
Molecular medicine and bioengineering
Avatar of 謝宗翰.
Avatar of 謝宗翰.
post doc @RIKEN IMS
2021 ~ Present
Data Analyst/Data Scientist
More than one year
one year under a fierce competition from Merck Millipore. - Successfully sold products to clients 3 months after entering the company. - Conducted cold call and joint call to develop clientsqPCR R&D assay • GeneReach Biotechnology corporation - Developed qPCR based IVD products for tuberculosis (TB) detectionInstrument and software FlowJo. GraphPad Prism. Fortessa. qPCR. ELISA. R. NGS data analysis. FACS. Flow cytometry analysis. Illumina. 10X Genomics. Education, Okinawa Institute of Science and Technology Graduate University, PhD Immunology, National Yang Ming University, MSc Immunology, National Yang Ming University, Bachelor, Life Sc
DNA Sequencing
RNAseq analysis
Flow Cytometry
Employed
Full-time / Interested in working remotely
6-10 years
Okinawa Institute of Science and Technology Graduate University
Immunology

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Within one month
Research assistant
Logo of National Yang Ming Chiao Tung University.
National Yang Ming Chiao Tung University
2021 ~ 2022
Johor, 馬來西亞
Professional Background
Current status
Employed
Job Search Progress
Open to opportunities
Professions
Researcher, Biotechnology Researcher
Fields of Employment
Pharmaceutical / Biotech
Work experience
1-2 years work experience (4-6 years relevant)
Management
I've had experience in managing 1-5 people
Skills
Research Skills
Data Analysis
Molecular Cloning
Recombinant protein engineering
Mammalian Cell Culture
Protein production by transfection
Protein production with Expi CHO system
Affinity Chromatography
SDS-PAGE
Western Blotting
ELISA
Animal experiment (mouse)
Animal model (collagen-induced arthritis)
Pharmacokinetics of protein drug
Primary cell isolation and culture
Languages
English
Intermediate
Chinese
Native or Bilingual
Malay
Beginner
Job search preferences
Positions
Researcher
Job types
Full-time
Locations
Singapore
Remote
Interested in working remotely
Freelance
No
Educations
School
國立陽明交通大學(National Yang Ming Chiao Tung University)
Major
Molecular medicine and bioengineering
Print

Tai Yuan Hong

Researcher | Malaysian | +65 87565790(Sg) | +60177529486(My) [email protected]

Work pass sponsor needed.

Summary

3 years experience in Immunology research, majorly in developing drugs for Rheumatoid Arthritis. Familiar with BSL2 lab protocol.  I am independent, self-motivated, and a good team player, capable of troubleshooting experiments and self-learning.

Working Experience

Research Assistant   •  National Yang Ming Chiao Tung University, Taiwan • 2021 - 2022

  1. Conducting experiments including:
    • Cloning (routine subcloning, PCR, Site-directed mutagenesis)
    • Mammalian Cell Culture (HEK293/HEK293T/CHO/Jurkat T cell/Raji B cell/CT26/RENCA/Hybridoma cell)
    • Protein/Antibody production by transient transfection
    • Protein/Antibody production by ExpiCHO/Expi293 expression system
    • Purification of protein/antibody by affinity chromatography column/FPLC
    • SDS-PAGE, Western blotting and ELISA
    • Fluorescent conjugated antibody staining and flow cytometry
    • Animal disease model (collagen-induced arthritis) and syngeneic mouse tumor model(CT26 and RENCA)
    • Primary cell isolation from mouse
    • Mouse tissue processing, section, and histology staining
  2. Familiar with C57BL/6, BALB/c, and DBA/1 mouse strains
  3. Breeding experimental animal (mouse)
  4. Data analysis
  5. Assisting paper writing


Protein Purification Researcher   •  Genscript Singapore • 2023 - Now

  1. Purification with Akta system
  2. Purification with magnetic beads
  3. Protein/Antibody purification by
    1. Protein A/G/L, CH1
    2. Nickel Purification 
    3. Ion exchange chromatography
    4. Size exclusive chromatography

Education


National Yang Ming Chiao Tung University, Taiwan

Master of Science, 2019 - 2021

Molecular medicine and Bioengineering

Thesis: Application of Alb-CTLA-4Ig in mouse collagen-induced arthritis


National Chiao Tung University, Taiwan

Bachelor of Science, 2015 - 2019

Biological science and technology



Publication

A lesion-selective albumin-CTLA4Ig as a safe and effective treatment for collagen-induced arthritis

Jiang FY*, Zhang YZ*, Tai YH*, Chou CY, Hsieh YC, Chang YC, Huang HC, Li ZQ, Hsieh YC, Chen IJ, Huang BC, Su YC, Lin WW, Lin HC, Chao JI, Yuan SF, Wang YM, Cheng TL, Tzou SC. (*Fu-Yao Jiang, Yan-Zhu Zhang, and Yuan-Hong Tai contributed equally to this study)

Inflammation and Regeneration 2023 Feb 16;43(1):13. 

doi: 10.1186/s41232-023-00264-8. 

Skills


  • Recombinant protein engineering
  • Animal experiment skills
  • Microsoft Office and Photoshop
  • Graphpad Prism software

Language


  • English - Fairly Good
  • Chinese - Fluent
  • Malay - Fairly Good
Resume
Profile

Tai Yuan Hong

Researcher | Malaysian | +65 87565790(Sg) | +60177529486(My) [email protected]

Work pass sponsor needed.

Summary

3 years experience in Immunology research, majorly in developing drugs for Rheumatoid Arthritis. Familiar with BSL2 lab protocol.  I am independent, self-motivated, and a good team player, capable of troubleshooting experiments and self-learning.

Working Experience

Research Assistant   •  National Yang Ming Chiao Tung University, Taiwan • 2021 - 2022

  1. Conducting experiments including:
    • Cloning (routine subcloning, PCR, Site-directed mutagenesis)
    • Mammalian Cell Culture (HEK293/HEK293T/CHO/Jurkat T cell/Raji B cell/CT26/RENCA/Hybridoma cell)
    • Protein/Antibody production by transient transfection
    • Protein/Antibody production by ExpiCHO/Expi293 expression system
    • Purification of protein/antibody by affinity chromatography column/FPLC
    • SDS-PAGE, Western blotting and ELISA
    • Fluorescent conjugated antibody staining and flow cytometry
    • Animal disease model (collagen-induced arthritis) and syngeneic mouse tumor model(CT26 and RENCA)
    • Primary cell isolation from mouse
    • Mouse tissue processing, section, and histology staining
  2. Familiar with C57BL/6, BALB/c, and DBA/1 mouse strains
  3. Breeding experimental animal (mouse)
  4. Data analysis
  5. Assisting paper writing


Protein Purification Researcher   •  Genscript Singapore • 2023 - Now

  1. Purification with Akta system
  2. Purification with magnetic beads
  3. Protein/Antibody purification by
    1. Protein A/G/L, CH1
    2. Nickel Purification 
    3. Ion exchange chromatography
    4. Size exclusive chromatography

Education


National Yang Ming Chiao Tung University, Taiwan

Master of Science, 2019 - 2021

Molecular medicine and Bioengineering

Thesis: Application of Alb-CTLA-4Ig in mouse collagen-induced arthritis


National Chiao Tung University, Taiwan

Bachelor of Science, 2015 - 2019

Biological science and technology



Publication

A lesion-selective albumin-CTLA4Ig as a safe and effective treatment for collagen-induced arthritis

Jiang FY*, Zhang YZ*, Tai YH*, Chou CY, Hsieh YC, Chang YC, Huang HC, Li ZQ, Hsieh YC, Chen IJ, Huang BC, Su YC, Lin WW, Lin HC, Chao JI, Yuan SF, Wang YM, Cheng TL, Tzou SC. (*Fu-Yao Jiang, Yan-Zhu Zhang, and Yuan-Hong Tai contributed equally to this study)

Inflammation and Regeneration 2023 Feb 16;43(1):13. 

doi: 10.1186/s41232-023-00264-8. 

Skills


  • Recombinant protein engineering
  • Animal experiment skills
  • Microsoft Office and Photoshop
  • Graphpad Prism software

Language


  • English - Fairly Good
  • Chinese - Fluent
  • Malay - Fairly Good