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Research Assistant @National Yang Ming Chiao Tung University2021 ~ 2022
・
Researcher
Within one month
Tai Yuan Hong Researcher | Malaysian |Sg) |My) | [email protected] Work pass sponsor needed. Summary 3 years experience in Immunology research, majorly in developing recombinant protein drugs for Rheumatoid Arthritis. Familiar with BSL2 lab protocol. I am independent, self-motivated, and a good team player, capable of troubleshooting experiments and self-learning. Working Experience Research Assistant • National Yang Ming Chiao Tung University, Taiwan •Protein engineering & Drug development Conducting experiments including: Cloning (routine subcloning, PCR, Site-directed mutagenesis) Mammalian Cell Culture (HEK293/HEK293T/CHO/Jurkat T cell/Raji B cell/
Employed
・
Open to opportunities
Full-time / Interested in working remotely
4-6 years
國立陽明交通大學(National Yang Ming Chiao Tung University)
・
Molecular medicine and bioengineering
研究助理 @中研院RNAiCore 核心設施2021 ~ 2023
・
生物科技研發人員、醫藥研發人員、生物學專業與研究人員、助理研究人員、研究助理
Within one year
後來因為遇到我的指導教授才踏入了生物技術的世界，在動物疫苗實驗室中學習到了許多重要的技術， 基礎分生技術(Form cloning to protein expression and purification)、q-PCR、ELISA、Cell culture (CHO, 293T, NS-1, Sf 9)、 Recombinant virus preparation及實驗動物操作。種種的經歷讓我有能力開發出類病毒顆粒疫苗並取得
Employed
Full-time / Not interested in working remotely
4-6 years
國立宜蘭大學National Ilan University
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Definition of Reputation Credits
Technical Skills
Specialized knowledge and expertise within the profession (e.g. familiar with SEO and use of related tools).
Problem-Solving
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Adaptability
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Within one month
Research assistant
National Yang Ming Chiao Tung University
・
2021 ~ 2022
Professional Background
Current status
Employed
Job Search Progress
Open to opportunities
Professions
Researcher, Biotechnology Researcher
Fields of Employment
Pharmaceutical / Biotech
Work experience
1-2 years work experience (4-6 years relevant)
Management
I've had experience in managing 1-5 people
Skills
Languages
English
・
Intermediate
Chinese
・
Native or Bilingual
Malay
・
Beginner
Job search preferences
Positions
Researcher
Job types
Full-time
Locations
Singapore
Remote
Interested in working remotely
Freelance
No
Educations
School
國立陽明交通大學(National Yang Ming Chiao Tung University)
Major
Molecular medicine and bioengineering
Tai Yuan Hong
Researcher | Malaysian | +65 87565790(Sg) | +60177529486(My) | [email protected]
Work pass sponsor needed.
Summary
3 years experience in Immunology research, majorly in developing recombinant protein drugs for Rheumatoid Arthritis. Familiar with BSL2 lab protocol. I am independent, self-motivated, and a good team player, capable of troubleshooting experiments and self-learning.
Working Experience
Research Assistant • National Yang Ming Chiao Tung University, Taiwan • 2021 - 2022
- Protein engineering & Drug development
- Conducting experiments including:
- Cloning (routine subcloning, PCR, Site-directed mutagenesis)
- Mammalian Cell Culture (HEK293/HEK293T/CHO/Jurkat T cell/Raji B cell/CT26/RENCA/Hybridoma cell)
- Protein/Antibody production by transient transfection
- Protein/Antibody production by ExpiCHO/Expi293 expression system
- Purification of protein/antibody by affinity chromatography column/FPLC
- SDS-PAGE, Western blotting and ELISA
- Fluorescent conjugated antibody staining and flow cytometry
- Animal disease model (collagen-induced arthritis) and syngeneic mouse tumor model(CT26 and RENCA)
- Primary cell isolation from mouse
- Mouse tissue processing, section, and histology staining
- Familiar with C57BL/6, BALB/c, and DBA/1 mouse strains
- Breeding experimental animal (mouse)
- Data analysis
- Assisting paper writing
Protein Purification Researcher • Genscript Singapore • 2023 - 2024 April
- Purification with Akta system/magnetic beads
- Protein/Antibody purification by Akta with
- Protein A/G/L, CH1
- Nickel Purification
- Ion exchange chromatography
- Size exclusive chromatography
Education
National Yang Ming Chiao Tung University, Taiwan
Master of Science, 2019 - 2021
Molecular medicine and Bioengineering
Thesis: Application of Alb-CTLA-4Ig in mouse collagen-induced arthritis
National Chiao Tung University, Taiwan
Bachelor of Science, 2015 - 2019
Biological science and technology
Publication
A lesion-selective albumin-CTLA4Ig as a safe and effective treatment for collagen-induced arthritis
Jiang FY*, Zhang YZ*, Tai YH*, Chou CY, Hsieh YC, Chang YC, Huang HC, Li ZQ, Hsieh YC, Chen IJ, Huang BC, Su YC, Lin WW, Lin HC, Chao JI, Yuan SF, Wang YM, Cheng TL, Tzou SC. (*Fu-Yao Jiang, Yan-Zhu Zhang, and Yuan-Hong Tai contributed equally to this study)
Inflammation and Regeneration 2023 Feb 16;43(1):13.
doi: 10.1186/s41232-023-00264-8.
Skills
- Recombinant protein engineering
- Animal experiment skills
- Microsoft Office and Photoshop
- Graphpad Prism software
Language
- English - Fairly Good
- Chinese - Fluent
- Malay - Fairly Good
Tai Yuan Hong
Researcher | Malaysian | +65 87565790(Sg) | +60177529486(My) | [email protected]
Work pass sponsor needed.
Summary
3 years experience in Immunology research, majorly in developing recombinant protein drugs for Rheumatoid Arthritis. Familiar with BSL2 lab protocol. I am independent, self-motivated, and a good team player, capable of troubleshooting experiments and self-learning.
Working Experience
Research Assistant • National Yang Ming Chiao Tung University, Taiwan • 2021 - 2022
- Protein engineering & Drug development
- Conducting experiments including:
- Cloning (routine subcloning, PCR, Site-directed mutagenesis)
- Mammalian Cell Culture (HEK293/HEK293T/CHO/Jurkat T cell/Raji B cell/CT26/RENCA/Hybridoma cell)
- Protein/Antibody production by transient transfection
- Protein/Antibody production by ExpiCHO/Expi293 expression system
- Purification of protein/antibody by affinity chromatography column/FPLC
- SDS-PAGE, Western blotting and ELISA
- Fluorescent conjugated antibody staining and flow cytometry
- Animal disease model (collagen-induced arthritis) and syngeneic mouse tumor model(CT26 and RENCA)
- Primary cell isolation from mouse
- Mouse tissue processing, section, and histology staining
- Familiar with C57BL/6, BALB/c, and DBA/1 mouse strains
- Breeding experimental animal (mouse)
- Data analysis
- Assisting paper writing
Protein Purification Researcher • Genscript Singapore • 2023 - 2024 April
- Purification with Akta system/magnetic beads
- Protein/Antibody purification by Akta with
- Protein A/G/L, CH1
- Nickel Purification
- Ion exchange chromatography
- Size exclusive chromatography
Education
National Yang Ming Chiao Tung University, Taiwan
Master of Science, 2019 - 2021
Molecular medicine and Bioengineering
Thesis: Application of Alb-CTLA-4Ig in mouse collagen-induced arthritis
National Chiao Tung University, Taiwan
Bachelor of Science, 2015 - 2019
Biological science and technology
Publication
A lesion-selective albumin-CTLA4Ig as a safe and effective treatment for collagen-induced arthritis
Jiang FY*, Zhang YZ*, Tai YH*, Chou CY, Hsieh YC, Chang YC, Huang HC, Li ZQ, Hsieh YC, Chen IJ, Huang BC, Su YC, Lin WW, Lin HC, Chao JI, Yuan SF, Wang YM, Cheng TL, Tzou SC. (*Fu-Yao Jiang, Yan-Zhu Zhang, and Yuan-Hong Tai contributed equally to this study)
Inflammation and Regeneration 2023 Feb 16;43(1):13.
doi: 10.1186/s41232-023-00264-8.
Skills
- Recombinant protein engineering
- Animal experiment skills
- Microsoft Office and Photoshop
- Graphpad Prism software
Language
- English - Fairly Good
- Chinese - Fluent
- Malay - Fairly Good