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Colorado State University

Ubiquitin and Cellular Regulation in

Stephen F.

Austin State University

Postdoctoral Fellow

Sarah Bollinger Martinez I have over five years of experience as an UI/UX designer. By incorporating data analysis and user interview, I have assis...

Sarah Bollinger Martinez

MERCK RESEARCH LABORATORIES

i.	 Identification of novel MHC-I presented antigens for cancer vaccine development 
 	 		 Developed a PROTAC-based screening platform for MHC-I presented antigen discovery 
 	 		 Established automated protocols for MHC-I immunoprecipitations in medium-throughput 
 	 		 Conducted immunopeptidomics studies to profile MHC-I presented peptides 
 	 ii.	 Screening for compounds that upregulate surface MHC-I expression for enhanced immunotherapies 
 		 Created an ELISA-based assay to assess the levels of surface MHC-I expression 
 		 Screened compound libraries to discover small molecules that enhance MHC-I antigen presentation 
 		 Proteotyped cells treated with MHC-I modulators to obtain mechanistic insights

i.	 Quantification of ubiquitin flux in live cells 
 	 		 Developed SILOW Stable Isotopic Labeling with 18O-Water method to quantify flux of 
 	 ubiquitination sites in yeast and human cells using LC-MS/MS 
 	 		 Made first observations of proteome-wide ubiquitination site turnover in cells 
 	 ii.	 Steady-state quantification of cellular ubiquitin in each major chemical form i.e. free, thioester - activated, substrate-conjugated 
 		 Created and optimized protocols to derivitize ubiquitin 
 		 Standardized fluorescence-based assays to quantify ubiquitin using an engineered binding protein

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