Academia Sinica is the most prestigious research institution in Taiwan. My roles include: • Reprogramming hiPSCs from human fibroblast via mRNA transfection. • Establishing and optimizing rapid differentiation protocol for dendritic cells from hiPSC to adapt the process onto an automated system. • Designing flow cytometry panel to validate cell phenotype. • Generating NK feeder cells through lentivirus packaging and transduction. • Stem cell culture.

Project 1: Investigation of a long non-coding RNA (lncRNA) X in Taiwanese colorectal cancer (CRC) patients. • Successfully generated lncRNA X misexpression stable cell lines by lentivirus transduction. • Conducted cell-based assays and tests to dissect the functional roles and significance of the lncRNA X in tumorigenesis. • Performed immunoprecipitation to investigate and analyse potential downstream interaction proteins. Project 2: Examined the involvement of microRNA in exosomes in epithelial-mesenchymal transition (EMT). • Harvested, quantified the size and number of exosomes via NanoSight NS300 and performed cell assays to study EMT. • Assisted in the subcutaneous injection of modified cancer cell lines and intraperitoneally administrated drugs in nude mice for drug resistance study. • Trained and supervised students in molecular biology techniques.

• Recruited study participants and explained the purpose of the study. • Processed human blood samples of STEMI patients for further experiments. • Ensured studies are conducted in accordance with ICH GCP and the guidelines of the Institutional Review Board (IRB). • Coordinated operations with different departments, including communication with site supervisors. • Managed information database of more than 400 patients via FileMaker.

• Promoted events and gatherings through social media. • Assisted in coordinating events. • Assisted in recruiting new members.

The branch has the most revenue of all branches in Macau. • Remained calm when working under pressure during busy weekend shifts. • Enhanced spoken communication skills by interacting with colleagues and customers. • Acquired multi-tasking and problem-solving skills. • Improved organisational skills through managing stock-taking.