In addition to basic molecular biology and cell biology experiments (such as Western blotting, qPCR, cell culture, etc.), I have also participated in animal experiments involving antibody preparation and the study of molecular mechanisms. I also have experience in culturing and differentiating placental stem cells and published a related research paper in the Journal of Biological Chemistry in 2018. Apart from designing experimental plans, I have also been involved in writing research papers and project applications (National Health Institute projects). After joining AIBIOS K.K. , I have not only expanded my expertise in immune cell culture and flow cytometry operation but have also contributed to the establishment of drug functional testing methods. 

SKills

1. Protein analysis: protein pull-down, Western blotting, BCA assay, ELISA, etc.

2. Virus packaging and collection: Preparation of lentivirus, retrovirus, and Zika virus in small quantities.

3. Plasmid construction

4. RNA-related analysis: RNA purification, qPCR, preparation of RNA sequencing samples.

5. Cell culture: Placental cells, placental stem cells, and immune cell culture.

6. Gene editing: CRISPR/Cas9 gene knockout.

7. Polyclonal antibody preparation and purification: Preparation of antigen peptides, experimental animal antigen injection, experimental animal blood collection, and serum separation.I wrote the thesis for this degree. The results for these years also published in Journal of Biological Chemistry in 2018 (Link: https://pubmed.ncbi.nlm.nih.gov/29743241/).

8. Flow cytometry analysis: Analysis of  NK cell -based methods.

1. At the university received three academic excellence awards.

2. Received Professor Chen Xisong's Analytical Chemistry Excellence Scholarship.

3. Published paper in JBC, Title:New insights into the regulation of placental growth factor gene expression by the transcription factors GCM1 and DLX3 in human placenta.