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Definition of Reputation Credits
SKILLS & RESEARCH EXPERIENCE
Laboratory skills
Flow cytometry │ Ultracentrifugation │ NTA │ Cell assays│ Lentivirus packaging and transduction│ Molecular cloning│ Colony PCR │ DNA & RNA extraction │PCR │RT-qPCR│ PBMC isolation│ Immunohistochemistry │ Immunoprecipitation│ Western Blotting
Data handling & IT
GraphPad Prism 7 │ FlowJo│BLAST search│ MS Word│ Excel│ Powerpoint│ Emsembl│UCSC Genome Browser
Research Assistant│Academia Sinica, Taiwan
Apr 2022 ~ Present
Academia Sinica is the most prestigious research institution in Taiwan. My roles include:
• Reprogramming hiPSCs from human fibroblast via mRNA transfection.
• Developing and optimizing rapid dendritic cell differentiation protocol from hiPSC.
• Improved haematopoietic stem cell differentiation efficiency by 22%.
• Designing flow cytometry panel to validate cell phenotype.
• Generating NK feeder cells through lentivirus packaging and transduction.
• Stem cell culture.
Research Assistant│Chang Gung University, Taiwan
Aug 2020 ~ Mar 2022
Project 1: Investigation of a long non-coding RNA (lncRNA) X in Taiwanese colorectal cancer (CRC) patients.
• Successfully generated lncRNA X misexpression stable cell lines by lentivirus transduction.
• Conducted cell-based assays and tests to dissect lncRNA X's functional roles and pathological significance in CRC umorigenesis.
• Performed immunoprecipitation to investigate and analyse potential downstream interaction proteins.
Project 2: Examined the involvement of microRNA in exosomes in epithelial-mesenchymal transition (EMT).
• Harvested, quantified the size and number of nanoparticles via NanoSight NS300 and performed cell assays to study EMT.
• Successfully built an exosome-treated lung adenocarcinoma EMT model.
• Assisted in the subcutaneous injection of modified cancer cell lines and intraperitoneally administrated drugs in nude mice to study the drug resistance of a particular lncRNA.
• Trained and supervised students in molecular biology techniques.
Research Assistant│Linkou Chang Gung Memorial Hospital, Taiwan
Dec 2019 ~ Aug 2020
• Processed human blood samples of STEMI patients through density gradient centrifugation for PBMC isolation for flow cytometry and molecular experiments.
• Recruited study participants, and established and maintained participant relationships.
• Managed projects, ensuring that each project meets the schedule.
• Ensured studies are conducted in accordance with ICH GCP and the guidelines of the Institutional Review Board.
• Coordinated operations with different departments, including communication with site supervisors.
• Managed information database of more than 400 patients via FileMaker.
Final Year Project│The Christie NHS Foundation Trust, UK
Feb ~ Apr 2019
Project title: "An investigation on the expression of caspase-X in DCIS and IDC in well-characterised patient cohorts".
• Identified of breast cancer biomarkers in 261 biopsy samples.
• Developed of an IHC protocol using caspase-X on the BenchMark ULTRA IHC/ ISH system.
• Analysed experimental data via SPSS.
• Executed accurate and timely record keeping and data management for each test undertaken.
Summer Research Intern│Academia Sinica, Taiwan
Jul ~ Aug 2018
• Shadowed supervisor's research project on Amyotrophic lateral sclerosis.
• Generated recombinant DNA for ADAR gene study via molecular cloning.
• Performed batch culture of bacteria, DNA isolation and purification.
• Conducted experiments such as PCR, gel electrophoresis, preparing culture media and Western blotting.
• Practised strict health and safety procedures in the laboratory, using the correct equipment appropriately and according to the task.
Education
The University of Manchester, BSc Hons in Biomedical Sciences
2016 ~ 2019
Core modules included: Molecular Biology, Biotechnology, Microbiology, Toxicology, Biochemistry, Bioinformatics & Pharmacology.
• Obtained upper-second class (2:1) degree.
• Awarded 1st class for 3 lab-based modules.
• Achieved 1st class in Toxicology and Microbiology.
• Achieved high 2:1 in Molecular Biology, Biotechnology & Bioinformatics.
ADDITIONAL SKILLS
Languages: English (Proficient - language of study)│ Cantonese (Native) │ Mandarin (Native)
Written communication skills developed through completing a dissertation, literature review and and writing up numerous laboratory projects.
Completed GCP and IRB courses provided by Center for Taiwan Academic Research Ethics Education.
Full clean driving licence.
SKILLS & RESEARCH EXPERIENCE
Laboratory skills
Flow cytometry │ Ultracentrifugation │ NTA │ Cell assays│ Lentivirus packaging and transduction│ Molecular cloning│ Colony PCR │ DNA & RNA extraction │PCR │RT-qPCR│ PBMC isolation│ Immunohistochemistry │ Immunoprecipitation│ Western Blotting
Data handling & IT
GraphPad Prism 7 │ FlowJo│BLAST search│ MS Word│ Excel│ Powerpoint│ Emsembl│UCSC Genome Browser
Research Assistant│Academia Sinica, Taiwan
Apr 2022 ~ Present
Academia Sinica is the most prestigious research institution in Taiwan. My roles include:
• Reprogramming hiPSCs from human fibroblast via mRNA transfection.
• Developing and optimizing rapid dendritic cell differentiation protocol from hiPSC.
• Improved haematopoietic stem cell differentiation efficiency by 22%.
• Designing flow cytometry panel to validate cell phenotype.
• Generating NK feeder cells through lentivirus packaging and transduction.
• Stem cell culture.
Research Assistant│Chang Gung University, Taiwan
Aug 2020 ~ Mar 2022
Project 1: Investigation of a long non-coding RNA (lncRNA) X in Taiwanese colorectal cancer (CRC) patients.
• Successfully generated lncRNA X misexpression stable cell lines by lentivirus transduction.
• Conducted cell-based assays and tests to dissect lncRNA X's functional roles and pathological significance in CRC umorigenesis.
• Performed immunoprecipitation to investigate and analyse potential downstream interaction proteins.
Project 2: Examined the involvement of microRNA in exosomes in epithelial-mesenchymal transition (EMT).
• Harvested, quantified the size and number of nanoparticles via NanoSight NS300 and performed cell assays to study EMT.
• Successfully built an exosome-treated lung adenocarcinoma EMT model.
• Assisted in the subcutaneous injection of modified cancer cell lines and intraperitoneally administrated drugs in nude mice to study the drug resistance of a particular lncRNA.
• Trained and supervised students in molecular biology techniques.
Research Assistant│Linkou Chang Gung Memorial Hospital, Taiwan
Dec 2019 ~ Aug 2020
• Processed human blood samples of STEMI patients through density gradient centrifugation for PBMC isolation for flow cytometry and molecular experiments.
• Recruited study participants, and established and maintained participant relationships.
• Managed projects, ensuring that each project meets the schedule.
• Ensured studies are conducted in accordance with ICH GCP and the guidelines of the Institutional Review Board.
• Coordinated operations with different departments, including communication with site supervisors.
• Managed information database of more than 400 patients via FileMaker.
Final Year Project│The Christie NHS Foundation Trust, UK
Feb ~ Apr 2019
Project title: "An investigation on the expression of caspase-X in DCIS and IDC in well-characterised patient cohorts".
• Identified of breast cancer biomarkers in 261 biopsy samples.
• Developed of an IHC protocol using caspase-X on the BenchMark ULTRA IHC/ ISH system.
• Analysed experimental data via SPSS.
• Executed accurate and timely record keeping and data management for each test undertaken.
Summer Research Intern│Academia Sinica, Taiwan
Jul ~ Aug 2018
• Shadowed supervisor's research project on Amyotrophic lateral sclerosis.
• Generated recombinant DNA for ADAR gene study via molecular cloning.
• Performed batch culture of bacteria, DNA isolation and purification.
• Conducted experiments such as PCR, gel electrophoresis, preparing culture media and Western blotting.
• Practised strict health and safety procedures in the laboratory, using the correct equipment appropriately and according to the task.
Education
The University of Manchester, BSc Hons in Biomedical Sciences
2016 ~ 2019
Core modules included: Molecular Biology, Biotechnology, Microbiology, Toxicology, Biochemistry, Bioinformatics & Pharmacology.
• Obtained upper-second class (2:1) degree.
• Awarded 1st class for 3 lab-based modules.
• Achieved 1st class in Toxicology and Microbiology.
• Achieved high 2:1 in Molecular Biology, Biotechnology & Bioinformatics.
ADDITIONAL SKILLS
Languages: English (Proficient - language of study)│ Cantonese (Native) │ Mandarin (Native)
Written communication skills developed through completing a dissertation, literature review and and writing up numerous laboratory projects.
Completed GCP and IRB courses provided by Center for Taiwan Academic Research Ethics Education.
Full clean driving licence.